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Literature summary for 6.1.1.12 extracted from

  • Fujiwara, S.; Lee, S.; Haruki, M.; Kanaya, S.; Takagi, M.; Imanaka, T.
    Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1 (1996), FEBS Lett., 394, 66-70.
    View publication on PubMed
Search for more literature for 6.1.1.12

Activating Compound

Activating Compound Comment Organism Structure
putrescine enhances activity, optimum concentrations is 0.07 mM Thermococcus kodakarensis
spermidine enhances activity, optimum concentrations is 0.07 mM Thermococcus kodakarensis
spermine enhances activity, optimum concentrations is 0.005 mM Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis
overexpression in Escherichia coli Pyrococcus sp.

General Stability

General Stability Organism
polyamines stabilize the conformation of the tRNA-AspRS complex Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 wild-type and N-terminal 32-residue truncated form (hDRS delta 32) expressed in Escherichia coli
-
Pyrococcus sp.
-
 overexpressed in Escherichia coli
-
Pyrococcus sp. KOD1
-
 overexpressed in Escherichia coli
-
Thermococcus kodakarensis Q52428
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus sp.
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Homo sapiens AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
GTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
GTP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
GTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 GMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
UTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
UTP + L-aspartate + tRNAAsp
-
 Thermococcus kodakarensis UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
UTP + L-aspartate + tRNAAsp
-
 Pyrococcus sp. KOD1 UMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
ASPA
-
 Thermococcus kodakarensis
Aspartyl-tRNA synthetase
-
 Thermococcus kodakarensis
AspRS
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Pyrococcus sp.
65
-
 optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine Thermococcus kodakarensis
75
-
 in presence of polyamine molecules, such as putrescine, spermine, and spermidine Pyrococcus sp.
75
-
 optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 75 35°C: about 60% of maximal activity, 75°C: about 50% of maximal activity Thermococcus kodakarensis
45 85 45°C: about 80% of maximal activity, 85°C: about 25% of maximal activity Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 no denaturation below Pyrococcus sp.
85
-
 the thermal melting temperature seems higher than 85°C in the presence of Mg2+. In the absence of Mg 2+, melting temperature decreases to 85°C Thermococcus kodakarensis
90
-
 half-life is 17 h Pyrococcus sp.
90
-
 half-life: 17 h Thermococcus kodakarensis
100
-
 half-life is 3.5 h Pyrococcus sp.
100
-
 half-life: 3.5 h Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pyrococcus sp.
7.5
-
-
 Thermococcus kodakarensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity Pyrococcus sp.
5.5 8 pH 5.5: about 60% of maximal activity, pH 8.0: about 65% of maximal activity Thermococcus kodakarensis