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Literature summary for 6.1.1.11 extracted from
- Purification and properties of seryl transfer ribonucleic acid synthetase from Escherichia coli (1970), J. Biol. Chem., 245, 923-930.
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes at low pH | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
L-Ser | pH 8.8 | Escherichia coli |  |
| 0.07 | - |
L-Ser | acylation | Escherichia coli | |
| 0.2 | - |
D-Ser | serine-dependent ATP-diphosphate exchange | Escherichia coli | |
| 0.3 | - |
L-Ser | serine-dependent ATP-diphosphate exchange | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity remains relatively unchanged between 5.2 and 24 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, sedimentation studies after dissociation in 6 M guanidine hydrochloride | Escherichia coli |
| 95000 | - |
sedimentation studies | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM potassium phosphate buffer, pH 6.8, containing 20 mM 2-mercaptoethanol, 0.5 mM EDTA and 10% glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-Ser + tRNASer | - |
Escherichia coli | ? | - |
? | |
| ATP + L-serine + tRNASer | D-Ser can replace L-Ser in ATP-diphosphate exchange | Escherichia coli | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| additional information | serine-dependent ATP-diphosphate exchange | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, sedimentation studies after dissociation in 6 M guanidine hydrochloride | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 8.8 | aminoacylation | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycerol stabilizes at low pH | Escherichia coli |
| 4 | 6 | 37°C, stability gradually decreases when pH is lowered from 6 to 4 | Escherichia coli |
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