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Literature summary for 6.1.1.11 extracted from

  • Holman, K.M.; Puppala, A.K.; Lee, J.W.; Lee, H.; Simonovic, M.
    Insights into substrate promiscuity of human seryl-tRNA synthetase (2017), RNA, 23, 1685-1699 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
tRNASer at pH 7.6, temperature not specified in the publication Homo sapiens
0.031
-
tRNASec at pH 7.6, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Homo sapiens
-
AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P49591
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography, Resource-Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASec 15.8% activity compared to tRNASer Homo sapiens AMP + diphosphate + L-seryl-tRNASec
-
?
ATP + L-serine + tRNASer
-
Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer 100% activity Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
?

Synonyms

Synonyms Comment Organism
SARS
-
Homo sapiens
SerRS
-
Homo sapiens
Seryl-tRNA synthetase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.2
-
tRNASec at pH 7.6, temperature not specified in the publication Homo sapiens
13.7
-
tRNASer at pH 7.6, temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
298
-
tRNASec at pH 7.6, temperature not specified in the publication Homo sapiens
1900
-
tRNASer at pH 7.6, temperature not specified in the publication Homo sapiens