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Literature summary for 6.1.1.11 extracted from

  • Wang, C.; Guo, Y.; Tian, Q.; Jia, Q.; Gao, Y.; Zhang, Q.; Zhou, C.; Xie, W.
    SerRS-tRNASec complex structures reveal mechanism of the first step in selenocysteine biosynthesis (2015), Nucleic Acids Res., 43, 10534-10545 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes E447K and E156T/R157S, sitting drop vapor diffusion method, using 12% (w/v) PEG 3350, 0.2 M NaCl and 0.1 MTris-HCl (pH 7.5) Homo sapiens

Protein Variants

Protein Variants Comment Organism
C117S the mutant shows increased activity compared to the wild type enzyme Homo sapiens
C46S the mutant shows increased activity compared to the wild type enzyme Homo sapiens
E156T/R157S the mutant retains about 10% of wild type activity Homo sapiens
E447K the mutant shows increased activity compared to the wild type enzyme Homo sapiens
G136V the mutant retains about 15% of wild type activity Homo sapiens
P30G31Y the mutation of the P30G31 dipeptide to a single tyrosine almost eliminates serylation activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GSSG about 16% residual activity at 1 mM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000 154000 dynamic light scattering Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Homo sapiens
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P49591
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Q-HP column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
 ?

Synonyms

Synonyms Comment Organism
SARS
-
 Homo sapiens
SerRS
-
 Homo sapiens
Seryl-tRNA synthetase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens