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Literature summary for 6.1.1.11 extracted from

  • Dulic, M.; Pozar, J.; Bilokapic, S.; Weygand-Durasevic, I.; Gruic-Sovulj, I.
    An idiosyncratic serine ordering loop in methanogen seryl-tRNA synthetases guides substrates through seryl-tRNASer formation (2011), Biochimie, 93, 1761-1769.
    View publication on PubMed
Search for more literature for 6.1.1.11

Protein Variants

Protein Variants Comment Organism
F397P/A399G the mutant shows deleterious effects in both kcat and KM values compared to the wild type enzyme Methanosarcina barkeri
G402A/G405A the double substitution displays an order of magnitude higher KM but only 2.5fold lower kcat Methanosarcina barkeri
H250A the mutant is highly efficient in serine activation: kcat is reduced by only 3fold, while KM is reduced relative to the wild type parameters Methanosarcina barkeri
H250A/Q400A the mutant is highly efficient in serine activation: kcat is reduced by only 3fold, while KM is reduced relative to the wild type parameters Methanosarcina barkeri
P395A the mutant shows 6.5fold reduced enzyme efficiency compared to the wild type Methanosarcina barkeri
Q400A the mutation produces only small kinetic effects: KM is elevated 2fold while kcat is reduced 2fold Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 L-serine wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.0257
-
 L-serine mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.049
-
 L-serine mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.0731
-
 L-serine mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.2491
-
 L-serine mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.9
-
 L-serine mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Methanosarcina barkeri
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Methanosarcina barkeri AMP + diphosphate + L-seryl-tRNASer
-
 ?

Synonyms

Synonyms Comment Organism
SerRS
-
 Methanosarcina barkeri
Seryl-tRNA synthetase
-
 Methanosarcina barkeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
 L-serine mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.016
-
 L-serine mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.15
-
 L-serine mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.38
-
 L-serine mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.41
-
 L-serine mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
1.06
-
 L-serine wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina barkeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 L-serine wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.0257
-
 L-serine mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.049
-
 L-serine mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.0731
-
 L-serine mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.2491
-
 L-serine mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri
0.9
-
 L-serine mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C Methanosarcina barkeri