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Literature summary for 6.1.1.11 extracted from

  • Lesjak, S.; Boko, D.; Weygand-Durasevic, I.
    Seryl-tRNA synthetases from methanogenic archaea: suppression of bacterial amber mutation and heterologous toxicity (2010), Food Technol. Biotechnol., 48, 512-518.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of the archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation Methanocaldococcus jannaschii
expression of wild type or mutant C315A archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation Methanococcus maripaludis

Protein Variants

Protein Variants Comment Organism
C315A site-directed mutagenesis Methanococcus maripaludis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanocaldococcus jannaschii
Mg2+ required Methanococcus maripaludis
Zn2+ catalytic zinc ion in the active site Methanocaldococcus jannaschii
Zn2+ catalytic zinc ion in the active site Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Methanocaldococcus jannaschii
-
AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer Methanococcus maripaludis
-
AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanococcus maripaludis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
Methanocaldococcus jannaschii AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer
-
Methanococcus maripaludis AMP + diphosphate + L-seryl-tRNASer
-
?

Subunits

Subunits Comment Organism
More the enzyme has a idiosyncratic N-terminal domain, structure analysis, overview Methanocaldococcus jannaschii
More the enzyme has a idiosyncratic N-terminal domain, structure analysis, overview Methanococcus maripaludis

Synonyms

Synonyms Comment Organism
SerRS
-
Methanocaldococcus jannaschii
SerRS
-
Methanococcus maripaludis
Seryl-tRNA synthetase
-
Methanocaldococcus jannaschii
Seryl-tRNA synthetase
-
Methanococcus maripaludis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Methanocaldococcus jannaschii
30
-
assay at Methanococcus maripaludis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanocaldococcus jannaschii
7.5
-
assay at Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Methanocaldococcus jannaschii
ATP
-
Methanococcus maripaludis

General Information

General Information Comment Organism
evolution although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae Methanocaldococcus jannaschii
evolution although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae Methanococcus maripaludis
additional information expression of methanogenic-type SerRSs is toxic for Escherichia coli cells Methanocaldococcus jannaschii
additional information expression of methanogenic-type SerRSs is toxic for Escherichia coli cells Methanococcus maripaludis