Cloned (Comment) | Organism |
---|---|
expression of the archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation | Methanocaldococcus jannaschii |
expression of wild type or mutant C315A archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation | Methanococcus maripaludis |
Protein Variants | Comment | Organism |
---|---|---|
C315A | site-directed mutagenesis | Methanococcus maripaludis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanocaldococcus jannaschii | |
Mg2+ | required | Methanococcus maripaludis | |
Zn2+ | catalytic zinc ion in the active site | Methanocaldococcus jannaschii | |
Zn2+ | catalytic zinc ion in the active site | Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | Methanococcus maripaludis | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanococcus maripaludis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | - |
Methanococcus maripaludis | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme has a idiosyncratic N-terminal domain, structure analysis, overview | Methanocaldococcus jannaschii |
More | the enzyme has a idiosyncratic N-terminal domain, structure analysis, overview | Methanococcus maripaludis |
Synonyms | Comment | Organism |
---|---|---|
SerRS | - |
Methanocaldococcus jannaschii |
SerRS | - |
Methanococcus maripaludis |
Seryl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Seryl-tRNA synthetase | - |
Methanococcus maripaludis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Methanocaldococcus jannaschii |
30 | - |
assay at | Methanococcus maripaludis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanocaldococcus jannaschii |
7.5 | - |
assay at | Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanocaldococcus jannaschii | |
ATP | - |
Methanococcus maripaludis |
General Information | Comment | Organism |
---|---|---|
evolution | although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae | Methanocaldococcus jannaschii |
evolution | although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae | Methanococcus maripaludis |
additional information | expression of methanogenic-type SerRSs is toxic for Escherichia coli cells | Methanocaldococcus jannaschii |
additional information | expression of methanogenic-type SerRSs is toxic for Escherichia coli cells | Methanococcus maripaludis |