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Literature summary for 6.1.1.11 extracted from

  • Fukui, H.; Hanaoka, R.; Kawahara, A.
    Noncanonical activity of seryl-tRNA synthetase is involved in vascular development (2009), Circ. Res., 104, 1253-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX6p3 for expression in Escherichia coli cells Danio rerio

Protein Variants

Protein Variants Comment Organism
additional information ko095, an embryonic lethal mutant with abnormal branching in the head and trunk blood vessels, the gene responsible for ko095 encodes seryl-tRNA synthetase with a nonsense mutation Danio rerio
T429A the abnormal branching of intersegmental vessels in ko095 mutant is suppressed by the introduction of either wild-type or mutant T429A seryl-tRNA synthetase, T429A lacks the enzymatic activity that catalyzes aminoacylation of transfer RNA for serine Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Danio rerio
-
AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

Organism UniProt Comment Textmining
Danio rerio Q6DRC0
-
-

Purification (Commentary)

Purification (Comment) Organism
by glutathione Sepharose beads Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
Danio rerio AMP + diphosphate + L-seryl-tRNASer
-
?

Synonyms

Synonyms Comment Organism
Seryl-tRNA synthetase
-
Danio rerio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
aminoacylation assay at room temperature Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
aminoacylation assay Danio rerio

Cofactor

Cofactor Comment Organism Structure
ATP
-
Danio rerio

General Information

General Information Comment Organism
physiological function seryl-tRNA synthetase is involved in vascular development Danio rerio