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Literature summary for 6.1.1.11 extracted from
- Characterization of two seryl-tRNA synthetases in albomycin-producing Streptomyces sp. strain ATCC 700974 (2009), Antimicrob. Agents Chemother., 53, 4619-4627.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vectors pET30a+ and pUC19 | Streptomyces sp. |
| into the vectors pET30a+, pSUP and pUC19, the H270G PCR product is cloned into the vector pET15b | Streptomyces sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H270G | SerRS2 mutant | Streptomyces sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SB-217452 | - |
Streptomyces sp. |  |
| serine hydroxamate | - |
Streptomyces sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0086 | - |
tRNASer | 37°C, aminoacylation | Streptomyces sp. | |
| 0.012 | - |
tRNASer | 30°C, aminoacylation | Streptomyces sp. | |
| 0.24 | - |
tRNASer | aminoacylation | Streptomyces sp. | |
| 0.26 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
| 0.4 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
| 0.41 | - |
tRNASer | H270G mutant, aminoacylation | Streptomyces sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | Streptomyces sp. | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | D0UD38 | strain ATCC 700974 | - |
| Streptomyces sp. | D0UD39 | strain ATCC 700974 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| carried out with Ni-nitriloacetic acid spin columns | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Streptomyces sp. | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SerRS1 | - |
Streptomyces sp. |
| SerRS2 | - |
Streptomyces sp. |
| seryl-tRNA synthetase 1 | - |
Streptomyces sp. |
| seryl-tRNA synthetase 2 | - |
Streptomyces sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
aminoacylation assay | Streptomyces sp. |
| 37 | - |
aminoacylation assay | Streptomyces sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
tRNASer | 30°C, aminoacylation | Streptomyces sp. | |
| 0.125 | - |
tRNASer | 37°C, aminoacylation | Streptomyces sp. | |
| 0.18 | - |
tRNASer | H270G mutant, aminoacylation | Streptomyces sp. | |
| 1.37 | - |
tRNASer | aminoacylation | Streptomyces sp. | |
| 1.8 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
| 7 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
aminoacylation assay | Streptomyces sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptomyces sp. | |
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