Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Saccharomyces cerevisiae |
expressed as a His-tagged fusion protein in Escherichia coli | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ZmcSerRS does not bind yeast Pex21 protein. Although the yeast C-terminal SerRS extension is required for Pex21p binding, the maize counterpart with an appended yeast SerRS extension remains incapable of Pex21p binding, implying that additional regions of yeast SerRS also contribute to the interaction with the peroxin | Zea mays |
ScSerRSDELTAC13 | mutant containing a deletion of 13 C-terminal residues, mutant is readily complement a ScSerRS null allele, Km and Kcat values comparable to wild-type, T50: 44.5°C, interaction with Pex21p is lost | Saccharomyces cerevisiae |
ScSerRSDELTAC203 | mutant containing a deletion of 20 C-terminal residues, mutant does not complement a ScSerRS null allele, T50: 44.5°C | Saccharomyces cerevisiae |
ZmcSerRSDELTAC12 | mutant containing a deletion of 12 C-terminal residues, mutant readily complements a yeast SerRS null allele, Km and Kcat values comparable to wild-type | Zea mays |
ZmcSerRSDELTAC18 | mutant containing a deletion of 18 C-terminal residues, mutant readily complements a yeast SerRS null allele, Km and Kcat values comparable to wild-type, T50: 31.5°C, interaction with Pex21p is lost | Zea mays |
ZmcSerRSDELTAC26 | mutant containing a deletion of 26 C-terminal residues, mutant does not complement a yeast SerRS null allele, enzymatic activity of mutant protein is comparable to negative control. Expression of mutant protein is probably severly hindered due to intrinsic instability of the truncated protein | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00058 | - |
tRNASer | wild-type, using maize tRNA | Zea mays | |
0.00065 | - |
tRNASer | mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA | Saccharomyces cerevisiae | |
0.00065 | - |
tRNASer | mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA | Zea mays | |
0.00066 | - |
tRNASer | mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA | Zea mays | |
0.00071 | - |
tRNASer | wild-type, using yeast tRNA | Saccharomyces cerevisiae | |
0.047 | - |
L-Ser | wild-type, using yeast tRNA | Saccharomyces cerevisiae | |
0.051 | - |
L-Ser | mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA | Saccharomyces cerevisiae | |
0.108 | - |
L-Ser | mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA | Zea mays | |
0.145 | - |
L-Ser | mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA | Zea mays | |
0.149 | - |
L-Ser | wild-type, using maize tRNA | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Zea mays | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | - |
Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCSerS | - |
Saccharomyces cerevisiae |
Seryl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Seryl-tRNA synthetase | - |
Zea mays |
ZmSerS | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
30 | - |
assay at | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ScSerRS (wild-type) T50: 31°C, ScSerRSDELTAC13 (deletion of 13 C-terminal residues) T50: 44.5°C, ScSerRSDELTAC20 (deletion of 20 C-terminal residues) T50: 44.5°. Enzyme destabilization occurs upon removal of the proximal region of the extension, rich in proline and hydrophobic amino acids, since ScSerRSDC20 displays markedly decreased thermal stability | Saccharomyces cerevisiae |
additional information | - |
ZmcSerRS (wild-type) T50: 31°C, ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues) T50: 31.5. This indicates that deletion of the major part of the extension comprising polar and charged amino acids does not affect enzyme structure and stability | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
L-Ser | wild-type, using yeast tRNA | Saccharomyces cerevisiae | |
0.47 | - |
L-Ser | mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA | Saccharomyces cerevisiae | |
0.54 | - |
tRNASer | mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA | Saccharomyces cerevisiae | |
0.58 | - |
tRNASer | wild-type, using yeast tRNA | Saccharomyces cerevisiae | |
1.28 | - |
tRNASer | mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA | Zea mays | |
1.43 | - |
tRNASer | mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA | Zea mays | |
1.48 | - |
tRNASer | wild-type, using maize tRNA | Zea mays | |
2.8 | - |
L-Ser | wild-type, using maize tRNA | Zea mays | |
3.2 | - |
L-Ser | mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA | Zea mays | |
3.7 | - |
L-Ser | mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
7.5 | - |
assay at | Zea mays |