Crystallization (Comment) | Organism |
---|---|
crystal structure analysis for identification of the amino acid substrate discrimination mechanism | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | calculated binding energies in the activated mode agrees with kinetic measurements of a covalent bond between the amino acid and ATP, quantitative computational analysis of amino acid binding, overview | Saccharomyces cerevisiae | |
additional information | - |
additional information | calculated binding energies in the activated mode agrees with kinetic measurements of a covalent bond between the amino acid and ATP, quantitative computational analysis of amino acid binding, overview | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | Thermus thermophilus | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Thermus thermophilus | P34945 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | - |
Thermus thermophilus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | formation of a seryl adenylate intermediate, mechanism of discrimination between cognate and noncognate amino acids, quantitative computational analysis, overview | Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | formation of a seryl adenylate intermediate, mechanism of discrimination between cognate and noncognate amino acids, quantitative computational analysis, overview | Thermus thermophilus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
additional information | threonine will compete with serine for formation of the activated intermediate while alanine and glycine will not compete significantly, overview, binding of ATP or seryl adenylate leads to the stabilization of a motif 2 loop that interacts with the tRNA acceptor stem | Thermus thermophilus | ? | - |
? | |
additional information | threonine will compete with serine for formation of the activated intermediate while alanineand glycine will not compete significantly, overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SerRS | - |
Saccharomyces cerevisiae |
SerRS | - |
Thermus thermophilus |
Seryl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Seryl-tRNA synthetase | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
ATP | binding of ATP leads to the stabilization of a motif 2 loop that interacts with the tRNA acceptor stem | Thermus thermophilus |