Cloned (Comment) | Organism |
---|---|
expression of the mitochondrial isozyme in an enzyme-deficient Saccharomyces cerevisiae strain, caused by gene disruption, leads to functional complementation, overexpression of the mitochondrial isozyme in Escherichia coli BL21(DE3) | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.0004 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.0005 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.00067 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.04 | - |
L-serine | mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C | Zea mays | |
0.45 | - |
L-serine | mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.5, 30°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmic isozyme | Zea mays | 5737 | - |
mitochondrion | mitochondrial isozyme SerZMm | Zea mays | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Zea mays | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? | |
additional information | Zea mays | phylogenetic comparison | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
2 isozymes, one mitochondrial and another cytoplasmic | - |
Purification (Comment) | Organism |
---|---|
recombinant mitochondrial isozyme from overexpression in Escherichia coli BL21(DE3), to homogeneity | Zea mays |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer | subtrate recognition mechanism | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | enzyme utilizes tRNA substrates from Zea mays, Saccharomyces cerevisiae, and Escherichia coli, recombinant mitochondrial isozyme | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | tRNASer from Escherichia coli | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | chloroplastic tRNASer from maize | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | mitochondrial tRNASer from maize | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | mitochondrial tRNASer from yeast subtype 2 | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
additional information | no activity with tRNASer from Arabidopsis thaliana, the enzyme also performs the ATP-diphosphate exchange reaction | Zea mays | ? | - |
? | |
additional information | phylogenetic comparison | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
62 kDa RNA-binding protein | - |
Zea mays |
Serine transfer RNA synthetase | - |
Zea mays |
Serine translase | - |
Zea mays |
Serine--tRNA ligase | - |
Zea mays |
SerRS | - |
Zea mays |
SerRSmt | - |
Zea mays |
SERSEC | - |
Zea mays |
Seryl-transfer ribonucleate synthetase | - |
Zea mays |
Seryl-transfer ribonucleic acid synthetase | - |
Zea mays |
Seryl-transfer RNA synthetase | - |
Zea mays |
Seryl-tRNA synthetase | - |
Zea mays |
Synthetase, seryl-transfer ribonucleate | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.5 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
0.9 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
1.2 | - |
L-serine | mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C | Zea mays | |
1.2 | - |
tRNASer | mitochondrial isozyme, pH 7.5, 30°C | Zea mays | |
6.9 | - |
L-serine | mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.2, 30°C | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Zea mays |