Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Rokov-Plavec, J.; Lesjak, S.; Landeka, I.; Mijakovic, I.; Weygand-Durasevic, I.
    Maize seryl-tRNA synthetase: specificity of substrate recognition by the organellar enzyme (2002), Arch. Biochem. Biophys., 397, 40-50.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the mitochondrial isozyme in an enzyme-deficient Saccharomyces cerevisiae strain, caused by gene disruption, leads to functional complementation, overexpression of the mitochondrial isozyme in Escherichia coli BL21(DE3) Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.0004
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.0005
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.00067
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.04
-
L-serine mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C Zea mays
0.45
-
L-serine mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.5, 30°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cytoplasmic isozyme Zea mays 5737
-
mitochondrion mitochondrial isozyme SerZMm Zea mays 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Zea mays
-
AMP + diphosphate + L-seryl-tRNASer
-
?
additional information Zea mays phylogenetic comparison ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
2 isozymes, one mitochondrial and another cytoplasmic
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mitochondrial isozyme from overexpression in Escherichia coli BL21(DE3), to homogeneity Zea mays

Reaction

Reaction Comment Organism Reaction ID
ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer subtrate recognition mechanism Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer enzyme utilizes tRNA substrates from Zea mays, Saccharomyces cerevisiae, and Escherichia coli, recombinant mitochondrial isozyme Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer tRNASer from Escherichia coli Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer chloroplastic tRNASer from maize Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer mitochondrial tRNASer from maize Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer mitochondrial tRNASer from yeast subtype 2 Zea mays AMP + diphosphate + L-seryl-tRNASer
-
?
additional information no activity with tRNASer from Arabidopsis thaliana, the enzyme also performs the ATP-diphosphate exchange reaction Zea mays ?
-
?
additional information phylogenetic comparison Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
62 kDa RNA-binding protein
-
Zea mays
Serine transfer RNA synthetase
-
Zea mays
Serine translase
-
Zea mays
Serine--tRNA ligase
-
Zea mays
SerRS
-
Zea mays
SerRSmt
-
Zea mays
SERSEC
-
Zea mays
Seryl-transfer ribonucleate synthetase
-
Zea mays
Seryl-transfer ribonucleic acid synthetase
-
Zea mays
Seryl-transfer RNA synthetase
-
Zea mays
Seryl-tRNA synthetase
-
Zea mays
Synthetase, seryl-transfer ribonucleate
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.5
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
0.9
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
1.2
-
L-serine mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C Zea mays
1.2
-
tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.9
-
L-serine mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.2, 30°C Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
Zea mays