Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.10 extracted from

  • Ojo, K.K.; Ranade, R.M.; Zhang, Z.; Dranow, D.M.; Myers, J.B.; Choi, R.; Nakazawa Hewitt, S.; Edwards, T.E.; Davies, D.R.; Lorimer, D.; Boyle, S.M.; Barrett, L.K.; Buckner, F.S.; Fan, E.; Van Voorhis, W.C.
    Brucella melitensis methionyl-tRNA-synthetase (MetRS), a potential drug target for brucellosis (2016), PLoS ONE, 11, e0160350 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Brucella melitensis

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with inhibitors, sitting drop vapor diffusion method, using 0.2 M ammonium nitrate, 20% (wv)PEG-3350 pH 7.5 or 25% (w/v) PEG-1500, 100 mM MMT buffer, pH 5.0 or 25% (w/v) PEG-1500, 7% (w/v) PCB pH 4.0 or 0.2 Mammonium acetate, 0.1 M sodium acetate trihydrate, pH 4.6, 30% (w/v) PEG-4000 Brucella melitensis

Inhibitors

Inhibitors Comment Organism Structure
2-[(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)amino]quinolin-4(1H)-one
-
 Brucella melitensis
N-(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)-N'-thiophen-3-ylurea
-
 Brucella melitensis
N-(3-[[(3-chloro-5-methoxyphenyl)methyl]amino]propyl)-N'-phenylurea
-
 Brucella melitensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Brucella melitensis
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet Brucella melitensis 2308
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Brucella melitensis Q2YQ76 biovar Abortus
-
Brucella melitensis 2308 Q2YQ76 biovar Abortus
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Brucella melitensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Brucella melitensis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet
-
 Brucella melitensis 2308 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA-synthetase
-
 Brucella melitensis
MetRS
-
 Brucella melitensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60.6
-
 melting temperature of unbound apoenzyme Brucella melitensis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Brucella melitensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000006
-
 at pH 7.9 and 37°C Brucella melitensis 2-[(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)amino]quinolin-4(1H)-one
0.000272
-
 at pH 7.9 and 37°C Brucella melitensis N-(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)-N'-thiophen-3-ylurea
0.001657
-
 at pH 7.9 and 37°C Brucella melitensis N-(3-[[(3-chloro-5-methoxyphenyl)methyl]amino]propyl)-N'-phenylurea