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Literature summary for 6.1.1.10 extracted from

  • Suzuki, H.; Kaneko, A.; Yamamoto, T.; Nambo, M.; Hirasawa, I.; Umehara, T.; Yoshida, H.; Park, S.Y.; Tamura, K.
    Binding properties of split tRNA to the C-terminal domain of methionyl-tRNA synthetase of Nanoarchaeum equitans (2017), J. Mol. Evol., 84, 267-278 .
    View publication on PubMed
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in BL21-Codon Plus(DE3)-RIL cells Nanoarchaeum equitans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate (pH 4.6) Nanoarchaeum equitans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Nanoarchaeum equitans
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Nanoarchaeum equitans Q74MZ1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Blue Sepharose 6 gel filtration Nanoarchaeum equitans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Nanoarchaeum equitans AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
additional information binding of the enzyme to the split 3'-half tRNA species is stronger than to that of the 5'-half species Nanoarchaeum equitans ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Nanoarchaeum equitans

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Nanoarchaeum equitans
MetRS
-
 Nanoarchaeum equitans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nanoarchaeum equitans