Cloned (Comment) | Organism |
---|---|
expressed in BL21-Codon Plus(DE3)-RIL cells | Nanoarchaeum equitans |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate (pH 4.6) | Nanoarchaeum equitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Nanoarchaeum equitans | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nanoarchaeum equitans | Q74MZ1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Blue Sepharose 6 gel filtration | Nanoarchaeum equitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Nanoarchaeum equitans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
additional information | binding of the enzyme to the split 3'-half tRNA species is stronger than to that of the 5'-half species | Nanoarchaeum equitans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Nanoarchaeum equitans |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Nanoarchaeum equitans |
MetRS | - |
Nanoarchaeum equitans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nanoarchaeum equitans |