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Literature summary for 6.1.1.10 extracted from
- Substrate-assisted and enzymatic pretransfer editing of nonstandard amino acids by methionyl-tRNA synthetase (2015), Biochemistry, 54, 5757-5765 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pyrococcus abyssi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| C-terminal domain, hanging drop vapor diffusion method, using 20% (w/v) PEG8000 as precipitating agent in the presence of 50 mM potassium phosphate | Pyrococcus abyssi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D666A | the mutant enzyme behaves as monomer | Pyrococcus abyssi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
ATP | mutant D666A, pH and temperature not specified in the publication | Pyrococcus abyssi |  |
| 0.064 | - |
L-methionine | mutant D666A, pH and temperature not specified in the publication | Pyrococcus abyssi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Pyrococcus abyssi | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | Q9V011 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Pyrococcus abyssi | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 84000, SDS-PAGE | Pyrococcus abyssi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methionyl-transfer RNA synthetase | - |
Pyrococcus abyssi |
| MetRS | - |
Pyrococcus abyssi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
the enzyme has a half-life time of 16 min at 51°C | Pyrococcus abyssi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
L-methionine | mutant D666A, pH and temperature not specified in the publication | Pyrococcus abyssi | |
| 23 | - |
ATP | mutant D666A, pH and temperature not specified in the publication | Pyrococcus abyssi | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pyrococcus abyssi | |
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Release 2023.1 (January 2023)
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