Cloned (Comment) | Organism |
---|---|
the unmutated monomeric versions of MetRS, truncated at residue 548, M547, is produced from the plasmid pBSM547, the pMTY21-sll plasmid codes for an N-terminally 6xHis-tagged M547 MetRS, leading to expression of the MetRS-SLL mutant | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of the mutated MetRS is determined in the apo form as well as complexed with methionine or azidonorleucine to 1.4 to 1.7 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L13S/Y260L/H301L | MetRS SLL-mutant with modified substrate specificity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Escherichia coli | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00959 | - |
- |
Purification (Comment) | Organism |
---|---|
the SLL-mutant is purified on columns containing Talon affinity resin and Q-Hiload resin, respectively | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + azidonorleucine + tRNAMet | MetRS SLL-mutant | Escherichia coli | AMP + diphosphate + azidonorleucinyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Escherichia coli |
MetRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |