Cloned (Comment) | Organism |
---|---|
into the vector pET101/D-TOPO, a His-Tag is attached, for expression in Escherichia coli BL21 StarDE3 cells | Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
the structure of methionyl-tRNA synthetase is determined to a resolution of 2.1 A | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
determined by SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Mycolicibacterium smegmatis | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R3E2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose is used, in addition gel filtration on a HiLoad 16/60 Superdex-200 column is applied | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Mycolicibacterium smegmatis |
MetRS | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycolicibacterium smegmatis |