Literature summary for 6.1.1.10 extracted from

Link, A.J.; Vink, M.K.; Agard, N.J.; Prescher, J.A.; Bertozzi, C.R.; Tirrell, D.A.
Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids (2006), Proc. Natl. Acad. Sci. USA, 103, 10180-10185.

Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged mutants in Escherichia coli strain XL-1 Blue	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
L13G	saturation mutagenesis, three mutant clones from screening of a saturation mutagenesis library, the mutants are capable of incorporating the long-chain amino acid azidonorleucine into recombinant proteins with modest efficiency	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + azidonorleucine + tRNAMet	Escherichia coli	activity of mutant L13G, overview	AMP + diphosphate + azidonorleucyl-tRNAMet	-	?
ATP + L-methionine + tRNAMet	Escherichia coli	-	AMP + diphosphate + L-methionyl-tRNAMet	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00959	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography	Escherichia coli
recombinnat His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + azidonorleucine + tRNAMet	activity of mutant L13G, overview	Escherichia coli	AMP + diphosphate + azidonorleucyl-tRNAMet	-	?
ATP + L-methionine + tRNAMet	-	Escherichia coli	AMP + diphosphate + L-methionyl-tRNAMet	-	?

Synonyms

Synonyms	Comment	Organism
MetRS	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)