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Literature summary for 6.1.1.10 extracted from
- Two forms of methionyl-tranfer RNA synthetase from Mycobacterium smegmatis (1976), Biochem. Biophys. Res. Commun., 71, 939-951.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.133 | - |
L-Met | ATP-diphosphate exchange, enzyme form B | Mycolicibacterium smegmatis |  |
| 0.166 | - |
L-Met | ATP-diphosphate exchange, enzyme form A | Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely dependent on | Mycolicibacterium smegmatis | |
| Mg2+ | optimal concentration: 10 mM | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
| 31000 | - |
2 * 31000, enzyme form A, SDS-PAGE | Mycolicibacterium smegmatis |
| 31000 | - |
2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
| 65000 | - |
enzyme form A, gel filtration | Mycolicibacterium smegmatis |
| 85000 | - |
enzyme form B, gel filtration | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two enzyme forms: A and B | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
enzyme form B | Mycolicibacterium smegmatis |
| 0.09 | - |
enzyme form A | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| additional information | no other amino acid than methionine is activated in ATP-diphosphate exchange | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | enzyme forms A and B show differences in their capacity to recognize the cognate tRNAs | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | methionine-dependent ATP-diphosphate exchange | Mycolicibacterium smegmatis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31000, enzyme form A, SDS-PAGE | Mycolicibacterium smegmatis |
| tetramer | 2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Mycolicibacterium smegmatis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.94 | - |
L-Met | ATP-diphosphate exchange, enzyme form A | Mycolicibacterium smegmatis | |
| 3.13 | - |
L-Met | ATP-diphosphate exchange, enzyme form B | Mycolicibacterium smegmatis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
sodium cacodylate or Tris buffer | Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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