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Literature summary for 6.1.1.10 extracted from

  • Schmitt, E.; Panvert, M.; Blanquet, S.; Mechulam, Y.
    Transition state stabilization by the `high' motif of class I aminoacyl-tRNA synthetases. The case of Escherichia coli methionyl-tRNA synthetase (1995), Nucleic Acids Res., 23, 4793-4798.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
G23A mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
G23P mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
H21N mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
H21Q mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
H24N mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
H24Q mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli
L22A mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant, with reduced catalytic efficience and lowered maximal rate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of mutant enzymes in ATP-diphosphate exchange reaction Escherichia coli
0.02
-
 Met strain 547, ATP-diphosphate exchange Escherichia coli
0.41
-
 ATP strain 547, ATP-diphosphate exchange Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 M547 and mutant enzymes: L22A variant, G23A variant, G23P variant, H21N variant, H21Q variant, H24N variant, and H24Q variant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover numbers of mutant enzymes Escherichia coli
additional information
-
 additional information in ATP-diphosphate exchange reaction Escherichia coli