Literature summary for 6.1.1.1 extracted from

Wei, Z.; Xu, Z.; Liu, X.; Lo, W.S.; Ye, F.; Lau, C.F.; Wang, F.; Zhou, J.J.; Nangle, L.A.; Yang, X.L.; Zhang, M.; Schimmel, P.
Alternative splicing creates two new architectures for human tyrosyl-tRNA synthetase (2016), Nucleic Acids Res., 44, 1247-1255 .

Cloned(Commentary)

Cloned (Comment)	Organism
tyrS, DNA and amino acid sequence determination and analysis, analysis of two closely related, internally deleted, splice variants of homodimeric human tyrosyl-tRNA synthetase (TyrRS). Detection of the TyrRS transcripts in the cytoplasmic and polyribosomal RNA is carried out by PCR using primers targeting the 5'-UTR/exon1 and exon5/exon6 regions of the TyrRS gene (FP and RP2). Recombinant expression of splice variants in Jurkat T, THP-1, or HEK-293T cells, and recombinant expression of splice variants as thioredoxin-His-tagged proteins in Escherichia coli strain BL21(DE3), removal of the tag by protease-3C, followed by gel filtration	Homo sapiens

Cloned (Comment)

Organism

tyrS, DNA and amino acid sequence determination and analysis, analysis of two closely related, internally deleted, splice variants of homodimeric human tyrosyl-tRNA synthetase (TyrRS). Detection of the TyrRS transcripts in the cytoplasmic and polyribosomal RNA is carried out by PCR using primers targeting the 5'-UTR/exon1 and exon5/exon6 regions of the TyrRS gene (FP and RP2). Recombinant expression of splice variants in Jurkat T, THP-1, or HEK-293T cells, and recombinant expression of splice variants as thioredoxin-His-tagged proteins in Escherichia coli strain BL21(DE3), removal of the tag by protease-3C, followed by gel filtration

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	two closely related, internally deleted, splice variants of homodimeric human tyrosyl-tRNA synthetase (TyrRS) are analyzed, in spite of both variants ablating a portion of the catalytic core and dimer-interface contacts of native TyrRS, each folded into a distinct stable structure. The internal deletion of TyrRSE2-4 splice variant gives an alternative, neomorphic dimer interface orthogonal to that of native TyrRS. One splice variant, TyrRSE2-4, is largely dimeric while the other is mostly monomeric. TyrRS SVs are specifically enriched in lymphocytes and lung tissue	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P54577	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant thioredoxin-His-tagged enzyme splice variants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	adult and fetal brain	Homo sapiens	-
leukocyte	peripheral blood leukocytes	Homo sapiens	-
lung	-	Homo sapiens	-
lymphocyte	-	Homo sapiens	-

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Tyrosyl-tRNA synthetase	-	Homo sapiens
TyrRS	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Homo sapiens