Any feedback?
Literature summary for 6.1.1.1 extracted from
- Twin attributes of tyrosyl-tRNA synthetase of Leishmania donovani a housekeeping protein translation enzyme and a mimic of host chemokine (2016), J. Biol. Chem., 291, 17754-17771 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tyrS, sequence comparisons, single copy gene, recombinant expression of N-terminally His6-tagged enzyme, overexpression of wild-type and mutant enzymes LdTyrRS | Leishmania donovani |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gene deletion mutations are attempted via targeted gene replacement. The heterozygous mutants show slower growth kinetics and exhibited attenuated virulence. LdTyrRS chromosomal null mutants do not survive. Single allele of the LdTyrRS gene is replaced in heterozygous mutant parasites (TyrRS/HYG or TyrRS/NEO), leading to 1.8fold decreased expression of TyrRS protein in heterozygous mutants (TyrRS/HYG) | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fisetin | inhibits the enzyme activity and the growth of promastigotes | Leishmania donovani |  |
| resveratrol | inhibits the enzyme activity and the growth of promastigotes | Leishmania donovani | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | LdTyrRS protein is present in the cytoplasm and exits from the parasite cytoplasm into the extracellular medium, presence of LdTyrRS in the host cytosol | Leishmania donovani | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Leishmania donovani | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tyrosine + tRNATyr | Leishmania donovani | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
| ATP + L-tyrosine + tRNATyr | Leishmania donovani BPK282A1 | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | E9BC28 | - |
- |
| Leishmania donovani BPK282A1 | E9BC28 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography, to homogeneity | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Leishmania donovani | - |
| promastigote | - |
Leishmania donovani | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tyrosine + tRNATyr | - |
Leishmania donovani | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
| ATP + L-tyrosine + tRNATyr | - |
Leishmania donovani BPK282A1 | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, about, recombinant N-terminally His6-tagged enzyme, SDS-PAGE, x * 74000, wild-type enzyme, SDS-PAGE | Leishmania donovani |
| More | domain organization of TyrRS, overview | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LdTyrRS | - |
Leishmania donovani |
| Tyrosyl-tRNA synthetase | - |
Leishmania donovani |
| TyrRS | - |
Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Leishmania donovani | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | domain organization of TyrRS from eukaryotes and prokaryotes, overview | Leishmania donovani |
| physiological function | aminoacyl-tRNA synthetases (aaRSs) are housekeeping enzymes essential for protein synthesis. Apart from their parent aminoacylation activity, several aaRSs perform non-canonical functions in diverse biological processes. Leishmania tyrosyl-tRNA synthetase (LdTyrRS) performs aminoacylation and acts as a mimic of host CXC chemokine. Non-canonical function of Leishmania donovani tyrosyl-tRNA synthetase. The enzyme is essential. The released, extracellular LdTyrRS functions as a neutrophil chemoattractant. LdTyrRS specifically binds to host macrophages with its ELR (Glu-Leu-Arg) peptide motif. The ELR-CXCR2 receptor interaction mediates this binding. This interaction triggers enhanced secretion of the proinflammatory cytokines TNF-alpha and interleukin-6 by host macrophages. Possible immunomodulating role of LdTyrRS in Leishmania infection. Triggering of cytokine secretion by LdTyrRS, overview | Leishmania donovani |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
