Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli TG2 cells | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Geobacillus stearothermophilus | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a Source 15Q-Sepharose anion-exchange column | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
TyrRS | - |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
a model is proposed, in which the KMSKS signature sequence is conformationally constrained and unable to participate in catalysis below 25°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | 8 | kinetic assays | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |