Cloned (Comment) | Organism |
---|---|
gene tyrZ, overexpression of the HIs-tagged enzyme in Escherichia coli strain JM105 | Acidithiobacillus ferrooxidans |
Protein Variants | Comment | Organism |
---|---|---|
H306A | no complementation of the thermosensitive Escherichia coli tyrS mutant HB2109, 3fold decrease in kcat for amino acid activation | Acidithiobacillus ferrooxidans |
H306D | no complementation of the thermosensitive Escherichia coli tyrS mutant HB2109 | Acidithiobacillus ferrooxidans |
H53A | no complementation of the thermosensitive Escherichia coli tyrS mutant HB2109, inactive | Acidithiobacillus ferrooxidans |
K395N | slight complementation of the thermosensitive Escherichia coli tyrS mutant HB2109, 17fold increase in Km for Escherichia coli tRNATyr, reduced activity | Acidithiobacillus ferrooxidans |
S356A | no complementation of the thermosensitive Escherichia coli tyrS mutant HB2109, 7fold increase in Km for Escherichia coli tRNATyr, reduced activity | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants | Acidithiobacillus ferrooxidans | |
0.0025 | - |
tRNATyr | aminoacylation reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
0.021 | - |
L-tyrosine | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
0.027 | - |
L-tyrosine | aminoacylation reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
0.07 | - |
ATP | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
0.13 | - |
ATP | aminoacylation reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, recombinant His-tagged enzyme, SDS-PAGE | Acidithiobacillus ferrooxidans |
105000 | - |
recombinant His-tagged enzyme, gel filtration | Acidithiobacillus ferrooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | Acidithiobacillus ferrooxidans | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant HIs-tagged enzyme from overexpression in Escherichia coli strain JM105 | Acidithiobacillus ferrooxidans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr | S356 and K395 play key roles in tRNA binding, H306, a residue at the junction of the catalytic and tRNA binding domains, stabilizes the Tyr-AMP:enzyme complex | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | - |
Acidithiobacillus ferrooxidans | AMP + Tyr-tRNATyr + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, recombinant His-tagged enzyme, SDS-PAGE | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
Tyrosine tRNA synthetase | - |
Acidithiobacillus ferrooxidans |
TyrRZ | - |
Acidithiobacillus ferrooxidans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants | Acidithiobacillus ferrooxidans | |
3.3 | - |
ATP | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
3.3 | - |
L-tyrosine | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
5.29 | - |
ATP | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
5.29 | - |
L-tyrosine | phosphate exchange reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
7.7 | - |
ATP | aminoacylation reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans | |
7.7 | - |
L-tyrosine | aminoacylation reaction, pH 8.0, wild-type enzyme | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acidithiobacillus ferrooxidans |