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Literature summary for 5.6.2.B2 extracted from

  • Haugland, G.; Rollor, C.; Birkeland, N.; Kelman, Z.
    Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum (2009), Extremophiles, 13, 81-88 .
    View publication on PubMed
Search for more literature for 5.6.2.B2

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is more active in Tris and Bicine buffers than Hepes and phosphate buffers Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ binding to ATP and to DNA is required for helicase activity Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HK10
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O the protein binds single-stranded DNA, has DNA-dependent ATPase activity and ATP-dependent 3'->5' helicase activity Thermoplasma acidophilum ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
minichromosome maintenance protein
-
 Thermoplasma acidophilum
Ta0799
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 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermoplasma acidophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
53 70 53°C: about 45% of maximal activity, 70°C: about % of maximal activity Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermoplasma acidophilum