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Literature summary for 5.6.2.2 extracted from

  • Bedez, C.; Lotz, C.; Batisse, C.; Broeck, A.; Stote, R.; Howard, E.; Pradeau-Aubreton, K.; Ruff, M.; Lamour, V.
    Post-translational modifications in DNA topoisomerase 2alpha highlight the role of a eukaryote-specific residue in the ATPase domain (2018), Sci. Rep., 8, 9272 .
No PubMed abstract available
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae and in BHK-21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K168A the mutant exhibits 7fold impaired DNA cleavage activity and no decatenation activity compared to the wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P11388
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylation acetylation of specific sites involved in the allosteric regulation of the enzyme provide a mechanism for modulation of its catalytic activity Homo sapiens
phosphoprotein
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + catenated kinetoplast DNA
-
 Homo sapiens ADP + phosphate + decatenated kinetoplast DNA
-
 ?
ATP + negatively supercoiled pUC19 DNA
-
 Homo sapiens ADP + phosphate + relaxed pUC19 DNA
-
 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase 2alpha
-
 Homo sapiens
Top2alpha
-
 Homo sapiens
type 2 DNA topoisomerase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49.2
-
 melting temperature of the wild type ATPase domain Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 ATP recombinant mutant enzyme K168A from Saccharomyces cerevisiae cells, at pH 7.9 and 30°C Homo sapiens
2.05
-
 ATP recombinant enzyme from Saccharomyces cerevisiae cells, at pH 7.9 and 30°C Homo sapiens
2.7
-
 ATP recombinant enzyme from BHK-21 cells, at pH 7.9 and 30°C Homo sapiens