Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | at least 2 mM ATP is required for optimal activity | African swine fever virus | |
dithiothreitol | the optimum concentration for enzyme activity is 1 mM | African swine fever virus |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | African swine fever virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the use of KCl is optimal at a concentration of 75 mM | African swine fever virus | |
Mg2+ | the enzyme requires Mg2+ at 6 mM for optimal activity. Magnesium can be substituted by Mn2+ (at least at 4 mM) or Cu2+ (at 2 mM) but not by Ca2+ or Zn2+ | African swine fever virus | |
Na+ | the use of NaCl is optimal at a concentration of 75 mM | African swine fever virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + ATP + H2O | African swine fever virus | - |
DNA + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
African swine fever virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catenated kDNA + ATP + H2O | - |
African swine fever virus | decatenated kDNA + ADP + phosphate | - |
? | |
DNA + ATP + H2O | - |
African swine fever virus | DNA + ADP + phosphate | - |
? | |
additional information | the enzyme cannot supercoil relaxed DNA | African swine fever virus | ? | - |
? | |
supercoiled pBR322 plasmid DNA + ATP + H2O | - |
African swine fever virus | relaxed pBR322 plasmid DNA + ADP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
pP1192R | - |
African swine fever virus |
type II DNA topoisomerase | - |
African swine fever virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
African swine fever virus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the optimal temperature for activity ranges between 30 and 37°C, and while 50°C still sustains some activity, no activity is observed below 20°C | African swine fever virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
African swine fever virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the activity of the enzyme is highest between pH 7.0 and 8.0, with 7.5 being optimal, but the enzyme is almost inactive at pH 9.0 | African swine fever virus |