Any feedback?
Literature summary for 5.6.2.2 extracted from
- DNA-binding activity of rat DNA topoisomerase II alpha C-terminal domain contributes to efficient DNA catenation in vitro (2016), J. Biochem., 159, 363-369 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FLAG-tagged enzyme is expressed in HEK-293 cells, GST-tagged enzyme C-terminal domain is expressed in Escherichia coli JM109 cells | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P41516 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose column chromatography | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| negatively supercoiled pUC18 plasmid DNA + ATP + H2O | - |
Rattus norvegicus | relaxed pUC18 plasmid DNA + ADP + phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA topoisomerase II alpha | - |
Rattus norvegicus |
| Topo IIalpha | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in proliferating cells, topoisomerase II activities of DNA catenation or decatenation are required for condensation of chromosomes and segregation of chromatids | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
