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Literature summary for 5.6.2.2 extracted from

  • Couturier, M.; Bizard, A.H.; Garnier, F.; Nadal, M.
    Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricus (2014), BMC Mol. Biol., 15, 18.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
132600
-
 TopR2, calculated Saccharolobus solfataricus
145700
-
 TopR1, calculated Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
negatively supercoiled DNA + ATP + H2O Saccharolobus solfataricus TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells positively supercoil DNA + ADP + phosphate
-
 ?
negatively supercoiled DNA + ATP + H2O Saccharolobus solfataricus P2 TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells positively supercoil DNA + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition actively dividing Sulfolobus solfataricus cells contain only small amounts of both reverse gyrases, approximately 50 TopR1 and 125 TopR2 molecules per cell at 80°C. Sulfolobus solfataricus cells are resistant at 45°C for several weeks, but there is neither cell division nor replication initiation; these processes are fully restored upon a return to 80°C. TopR1 is not found after three weeks at 45°C whereas the amount of TopR2 remains constant Saccharolobus solfataricus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled DNA + ATP + H2O
-
 Saccharolobus solfataricus positively supercoil DNA + ADP + phosphate
-
 ?
negatively supercoiled DNA + ATP + H2O TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells Saccharolobus solfataricus positively supercoil DNA + ADP + phosphate
-
 ?
negatively supercoiled DNA + ATP + H2O
-
 Saccharolobus solfataricus P2 positively supercoil DNA + ADP + phosphate
-
 ?
negatively supercoiled DNA + ATP + H2O TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells Saccharolobus solfataricus P2 positively supercoil DNA + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Reverse gyrase Sulfolobus solfataricus, like most Crenarchaeota, contains two genes each encoding a reverse gyrase, TopR1 and TopR2 Saccharolobus solfataricus
TopR1
-
 Saccharolobus solfataricus
TopR2
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 TopR1 is not active at 45°C but that TopR2 exhibits highly positive DNA supercoiling activity at 45°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the two reverse gyrases of Sulfolobus solfataricus are differently regulated, in terms of protein abundance, in vivo at 80°C and 45°C. TopR2 is present both at high and low temperatures and is therefore presumably required whether cells are dividing or not. By contrast, TopR1 is present only at high temperature where the cell division occurs, suggesting that TopR1 is required for controlling DNA topology associated with cell division activity and/or life at high temperature Saccharolobus solfataricus