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Literature summary for 5.6.2.2 extracted from

  • Buhler, C.; Lebbink, J.H.; Bocs, C.; Ladenstein, R.; Forterre, P.
    DNA topoisomerase VI generates ATP-dependent double-strand breaks with two-nucleotide overhangs (2001), J. Biol. Chem., 276, 37215-37222.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of both subunits in Escherichia coli Saccharolobus shibatae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45055
-
 2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence Saccharolobus shibatae
60528
-
 2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence Saccharolobus shibatae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + ATP + H2O Saccharolobus shibatae the enzyme generates ATP-dependent double-strand breaks with two-nucleotide overhangs on supercoiled or linear DNA. topoVI is covalently attached to the 5'-ends of the broken DNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae O05207 and O05208 O05207: subunit B, O05208: subunit A
-

Purification (Commentary)

Purification (Comment) Organism
purification procedure of a fully soluble recombinant topoVI is developed by expressing both subunits simultaneously in Escherichia coli Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + ATP + H2O the enzyme generates ATP-dependent double-strand breaks with two-nucleotide overhangs on supercoiled or linear DNA. topoVI is covalently attached to the 5'-ends of the broken DNA Saccharolobus shibatae ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence Saccharolobus shibatae

Synonyms

Synonyms Comment Organism
DNA topoisomerase VI
-
 Saccharolobus shibatae
topoVI
-
 Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Saccharolobus shibatae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus shibatae

Cofactor

Cofactor Comment Organism Structure
ATP the formation of the double-strand breaks on supercoiled or linear DNA is strictly dependent on the presence of ATP or adenosine 5'-(beta,gamma-imino)triphosphate. ATP binding is required to stabilize an enzyme conformation able to cleave the DNA backbone Saccharolobus shibatae