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Literature summary for 5.6.2.2 extracted from

  • Valenti, A.; Perugino, G.; Nohmi, T.; Rossi, M.; Ciaramella, M.
    Inhibition of translesion DNA polymerase by archaeal reverse gyrase (2009), Nucleic Acids Res., 37, 4287-4295.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0771
-
 ATP 80°C, pH not specified in the publication, wild-type enzyme Saccharolobus solfataricus
0.256
-
 ATP 80°C, pH not specified in the publication, mutant enzyme Y965F Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + ATP + H2O Saccharolobus solfataricus reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity. It is typical of microorganisms living at high temperature and might play a role in maintenance of genome stability and repair ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + ATP + H2O reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity. It is typical of microorganisms living at high temperature and might play a role in maintenance of genome stability and repair Saccharolobus solfataricus ?
-
 ?
DNA + ATP + H2O reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity Saccharolobus solfataricus ?
-
 ?

Synonyms

Synonyms Comment Organism
Reverse gyrase
-
 Saccharolobus solfataricus
TopR1
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.55
-
 ATP 80°C, pH not specified in the publication, wild-type enzyme Saccharolobus solfataricus
3.97
-
 ATP 80°C, pH not specified in the publication, mutant enzyme Y965F Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity. It is typical of microorganisms living at high temperature and might play a role in maintenance of genome stability and repair. Reverse gyrase inhibits DNA polymerase PolB1. Inhibition of PolY activity depends on both ATPase and topoisomerase activities of reverse gyrase, suggesting that the intact positive supercoiling activity is required for PolY inhibition. In vivo, reverse gyrase and PolY are degraded after induction of DNA damage. Inhibition by reverse gyrase and degradation might act as a double mechanism to control DNA polymerase PolY and prevent its potentially mutagenic activity when undesired. Inhibition of a translesion polymerase by topoisomerase-induced modification of DNA structure may represent a mechanism of regulation of these enzymes Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 ATP 80°C, pH not specified in the publication, mutant enzyme Y965F Saccharolobus solfataricus
0.033
-
 ATP 80°C, pH not specified in the publication, wild-type enzyme Saccharolobus solfataricus