Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
sitting drop method, crystal structure of the enzyme in the presence and absence of ATP | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29238 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | positively supercoils DNA, the enzyme requires ATP, which is bound and hydrolysed by a helicase-like domain | Archaeoglobus fulgidus | positively supercoiled DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
type I topoisomerase | - |
Archaeoglobus fulgidus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme appears to be unique to hyperthermophiles, where its activity is believed to protect the genome from denaturation | Archaeoglobus fulgidus |