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Literature summary for 5.6.2.2 extracted from
- Tyrosine 656 in topoisomerase IIbeta is important for the catalytic activity of the enzyme: identification based on artifactual +80-Da modification at this site (2011), Proteomics, 11, 829-842.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant topo IIbeta proteins in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutation of only Tyr656, but not Ser1395, Thr1326 or Ser1545, decreases topo IIbeta decatenation activity | Homo sapiens |
| S1395A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
| S1545A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
| T1426A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
| T1426A/S1545A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
| Y656F | site-directed mutagenesis, phosphorylation site mutant that shows reduced decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozymes topo IIalpha and beta | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | both human isozymes topo IIalpha and beta are phosphorylated. Phosphorylation sites of topo IIbeta are Tyr656, Ser1395, Thr1426 and Ser1545 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant topo IIbeta proteins from Saccharomyces cerevisiae by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HL-60 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate for topo IIbeta is kinetoplast DNA. Topoisomerase II catalyzes topological changes in DNA and decatenation reaction. Residue Tyr656 of topoisomerase IIbeta is important for its catalytic activity, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology modeling of the frame 449-1108 of topo IIbeta comprising Tyr656 and Tyr711 | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topo II | - |
Homo sapiens |
| Topo IIalpha | - |
Homo sapiens |
| Topo IIbeta | - |
Homo sapiens |
| Topoisomerase II | - |
Homo sapiens |
| topoisomerase IIbeta | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Topoisomerase II catalyzes topological changes in DNA | Homo sapiens |
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Release 2023.1 (January 2023)
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