Cloned (Comment) | Organism |
---|---|
expression of the enzyme in a two-hybrid system with Mod(mdg4)2.2 proteins | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | downregulation of Topo II by RNAi or mutations in the Top2 gene, knockout mutant phenotypes, overview. The Top2 RNAi knockdown affects gypsy phenotypes | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromosome | Topo II and insulator proteins do not co-localize on polytene chromosomes | Drosophila melanogaster | 5694 | - |
nucleus | - |
Drosophila melanogaster | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | Topo II and Mod(mdg4)2.2 proteins directly interact | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
gene Top2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Topo II and Mod(mdg4)2.2 proteins directly interact | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase II | - |
Drosophila melanogaster |
topo II | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | downregulation of Topo II by RNAi or mutations in the Top2 gene result in disruption of Su(Hw) insulator function, mediated by the Mod(mdg4)2.2 protein, which is a unique component of the Su(Hw) insulator complex. Mutations in Top2 cause a slight decrease of Mod(mdg4)2.2 transcript but have a dramatic effect on Mod(mdg4)2.2 protein levels. Mod(mdg4)2.2 is not present at insulator sites in Top2 mutants, while Su(Hw) is present | Drosophila melanogaster |
physiological function | Topo II is required to prevent Mod(mdg4)2.2 degradation and, consequently, to stabilize Su(Hw) insulator-mediated chromatin organization | Drosophila melanogaster |