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Literature summary for 5.6.2.2 extracted from

  • Sunter, N.J.; Cowell, I.G.; Willmore, E.; Watters, G.P.; Austin, C.A.
    Role of topoisomerase IIalpha in DNA damage response following IR and etoposide (2010), J. Nucleic Acids, 2010, pii: 710589.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Inhibitors

Inhibitors Comment Organism Structure
etoposide etoposide triggers the formation of topoisomerase II-DNA adducts. Enzyme-associated DNA breaks can be stabilised by drugs such as etoposide. The proteasome has a role in converting etoposide-stabilised protein-DNA complexes into frank double-strand breaks Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of formation and removal of topoisomerase II-DNA complexes in response to ionising radiation or etoposide treatment, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus topoisomerase II is an enzyme that alters the topological state of DNA via a transient covalent enzyme-bridged double strand break in the DNA, through which a second DNA helix can pass ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 two isoforms of topoisomerase II, termed alpha and beta
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic fibroblast cell line
-
 Mus musculus
-
mTOP2beta-4 cell wild-type mouse embryonic fibroblasts Mus musculus
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mtop2beta-5 cell immortalized mouse fibroblasts lacking topoisomerase IIbeta Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information topoisomerase II is an enzyme that alters the topological state of DNA via a transient covalent enzyme-bridged double strand break in the DNA, through which a second DNA helix can pass Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
topoisomerase IIalpha
-
 Mus musculus
topoisomerase IIbeta
-
 Mus musculus

General Information

General Information Comment Organism
additional information ionising radiation does not influence enzyme-DNA complexes and does not produce topoisomerase II-DNA adducts, overview. Topoisomerase II-DNA complexes inwild-type cells but not in TOP2beta-/- cells increase significantly at 24 hours with the proteasome inhibitor MG132, suggesting topoisomerase IIbeta adducts are removed by the proteasome Mus musculus