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Literature summary for 5.6.2.2 extracted from

  • Forterre, P.; Mirambeau, G.; Jaxel, C.; Nadal, M.; Duguet, M.
    High positive supercoiling in vitro catalyzed by an ATP and polyethylene glycol-stimulated topoisomerase from Sulfolobus acidocaldarius (1985), EMBO J., 4, 2123-2128.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
adenosine 5'-O-(3-thiotriphosphate) nonhydrolyzable ATP analogue sustains production of positive supercoils, but to a lesser extent, suggesting that ATP hydrolysis is necessary for efficient activity Sulfolobus acidocaldarius
ATP 0.005-0.01 mM are sufficient to support full positive supercoiling Sulfolobus acidocaldarius
Polyethylene glycol in presence of PEG 6000 the reaction becomes very fast and highly processive Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
K+ ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ Sulfolobus acidocaldarius
Mg2+ ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13200
-
glycerol gradient sedimentation Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled DNA + ATP + H2O the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA Sulfolobus acidocaldarius positively supercoiled DNA + ADP + phosphate
-
?
relaxed DNA + ATP + H2O the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA Sulfolobus acidocaldarius positively supercoiled DNA + ADP + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
ATP-dependent positive supercoiling Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Sulfolobus acidocaldarius