Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-O-(3-thiotriphosphate) | nonhydrolyzable ATP analogue sustains production of positive supercoils, but to a lesser extent, suggesting that ATP hydrolysis is necessary for efficient activity | Sulfolobus acidocaldarius | |
ATP | 0.005-0.01 mM are sufficient to support full positive supercoiling | Sulfolobus acidocaldarius | |
Polyethylene glycol | in presence of PEG 6000 the reaction becomes very fast and highly processive | Sulfolobus acidocaldarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ | Sulfolobus acidocaldarius | |
Mg2+ | ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13200 | - |
glycerol gradient sedimentation | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
negatively supercoiled DNA + ATP + H2O | the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA | Sulfolobus acidocaldarius | positively supercoiled DNA + ADP + phosphate | - |
? | |
relaxed DNA + ATP + H2O | the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA | Sulfolobus acidocaldarius | positively supercoiled DNA + ADP + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
ATP-dependent positive supercoiling | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Sulfolobus acidocaldarius |