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Literature summary for 5.6.2.2 extracted from

  • Suda, N.; Ito, Y.; Imai, T.; Kikumori, T.; Kikuchi, A.; Nishiyama, Y.; Yoshida, S.; Suzuki, M.
    The alpha4 residues of human DNA topoisomerase IIalpha function in enzymatic activity and anticancer drug sensitivity (2004), Nucleic Acids Res., 32, 1767-1773.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in drug-permeable yeast strain JN394t2-4 and in the temperature-sensitive strain SD1-4 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A772P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, about 10% activity compared to the recombinant wild-type enzyme in strain JN394t2-4 Homo sapiens
F775P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, inactive mutant Homo sapiens
H759A site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, the mutant shows reduced decatenation activity and amounts of etoposide-induced cleavable complexes compared to the wild-type enzyme Homo sapiens
H759P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, the mutant shows about 50% reduced decatenation activity and amounts of etoposide-induced cleavable complexes compared to the wild-type enzyme Homo sapiens
I769P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, nearly inactive mutant Homo sapiens
L771P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, nearly inactive mutant Homo sapiens
M762P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, mutant activity is similar to the wild-type activity Homo sapiens
M765P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, nearly inactive mutant Homo sapiens
M766P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, inactive mutant Homo sapiens
N770P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, the mutant shows 30% reduced decatenation activity and amounts of etoposide-induced cleavable complexes compared to the wild-type enzyme Homo sapiens
N774P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, mutant activity is similar to the wild-type activity Homo sapiens
Q773P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, mutant activity is similar to the wild-type activity Homo sapiens
T767P site-directed mutagenesis, mutation of a residue of the alpha-helix region responsible for anti-cancer drug sensitivity, mutant activity is similar to the wild-type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
doxorubicin anti-cancer drug Homo sapiens
etoposide anti-cancer drug Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasmid DNA pRYG + ATP + H2O cleavage of plasmid DNA pRYG Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
DNA topoisomerase IIalpha
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens