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Literature summary for 5.6.2.2 extracted from
- Functional compatibility between isoform alpha and beta of type II DNA topoisomerase (2004), J. Cell Sci., 117, 1047-1054.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| silencing of the enzyme by transfection of HeLA cells with RNA duplexes of 21 nucleotides in length for each of the isozymes alpha and beta, i.e alpha siRNA-1, alpha siRNA-2, beta siRNA-1, and beta siRNA-2, transfection results in specific silencing of isozyme IIalpha, not of isozyme IIbeta | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | isozyme IIalpha knockdown chromosomes are condensed and aligned at metaphase and are still segregated at anaphase, which is arrested if both isozymes are removed, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme is essential for controlling the conformation of both DNA and whole chromosomes, e.g. for shortening of the chromosome axes, isozyme IIbeta can partially substitute for isozyme IIalpha | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
two isozymes IIalpha and IIbeta | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is essential for controlling the conformation of both DNA and whole chromosomes, e.g. for shortening of the chromosome axes, isozyme IIbeta can partially substitute for isozyme IIalpha | Homo sapiens | ? | - |
? | |
| additional information | activity is measured using the decatenation assay with kinetoplast DNA as a substrate | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TOPOII | - |
Homo sapiens |
| type II DNA topoisomerase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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