Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.2 extracted from

  • Lindsey, R.H., Jr.; Bender, R.P.; Osheroff, N.
    Effects of benzene metabolites on DNA cleavage mediated by human topoisomerase II alpha: 1,4-hydroquinone is a topoisomerase II poison (2005), Chem. Res. Toxicol., 18, 761-770.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,4-benzoquinone a topoisomerase II poison in vivo and in vitro, enhances cleavage activity, inhibits ligation activity, at 0.025 mM, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone Homo sapiens
1,4-hydroquinone physiological precursor of 1,4-benzoquinone, a topoisomerase II poison, enhances cleavage activity, inhibits ligation activity, at 0.25 mM, alters the enzyme's cleavage site specificity, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,4-benzoquinone a topoisomerase II poison in vivo and in vitro, enhances cleavage activity, inhibits ligation activity, at 0.025 mM, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone Homo sapiens
1,4-hydroquinone physiological precursor of 1,4-benzoquinone, a topoisomerase II poison, enhances cleavage activity, inhibits ligation activity, at 0.25 mM, alters the enzyme's cleavage site specificity, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone Homo sapiens
etoposide
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for DNA ligation Homo sapiens
KCl required for DNA binding Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + ATP + H2O ATP-dependent breakage, passage, and rejoining of double-stranded DNA Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
topoisomerase II alpha
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens