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Literature summary for 5.6.2.2 extracted from
- Characterization of human NSCLC cell line with innate etoposide-resistance mediated by cytoplasmic localization of topoisomerase II alpha (2005), Cancer Sci., 96, 774-783.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the isozyme alpha is a target for many chemotherapeutic agents in clinical use | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| camptothecin | - |
Homo sapiens |  |
| doxorubicin | - |
Homo sapiens | |
| etoposide | - |
Homo sapiens | |
| additional information | cytoplasmic localization of the enzyme is a mechanism to confer drug resistance to cells, cytotoxic affects of drugs on different cell lines, overview | Homo sapiens | |
| Teniposide | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | INER-37 cells | Homo sapiens | 5737 | - |
| additional information | cytoplasmic localization of the enzyme is a mechanism to confer drug resistance to cells | Homo sapiens | - |
- |
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65460 | - |
x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE | Homo sapiens |
| 160000 | - |
x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE | Homo sapiens |
| 170000 | - |
x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
| INER-37 cell | non-small-cell lung cancer cell line, cell line with innate etoposide-resistance mediated by cytoplasmic localization of topoisomerase II alpha, 2 enzyme forms, a large and a small one, overview | Homo sapiens | - |
| INER-51/PSC-1 cell | lung cancer cell line | Homo sapiens | - |
| lung cancer cell line | VP-1 cell | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| theta-174 phage DNA + ATP + H2O | unknotting of theta-174 phage DNA | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topo II alpha | - |
Homo sapiens |
| topoisomerase II alpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
DNA unknotting assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
DNA unknotting assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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