Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a 42% content in viral DNA | Chlorella virus Marburg | |
additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a high content in viral DNA | Paramecium bursaria Chlorella virus 1 | |
N6-Methyladenine | i.e.6 mA, inhibits DNA scission by the enzyme, 10% content in viral DNA | Chlorella virus Marburg | |
N6-Methyladenine | i.e.6mA, inhibits DNA scission by the enzyme, high content in viral DNA | Paramecium bursaria Chlorella virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | DNA cleavage kinetics | Paramecium bursaria Chlorella virus 1 | |
additional information | - |
additional information | DNA cleavage kinetics | Chlorella virus Marburg |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Paramecium bursaria Chlorella virus 1 | |
KCl | - |
Chlorella virus Marburg | |
Mg2+ | required for cleavage | Paramecium bursaria Chlorella virus 1 | |
Mg2+ | required for cleavage | Chlorella virus Marburg | |
NaCl | - |
Paramecium bursaria Chlorella virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + ATP + H2O | Paramecium bursaria Chlorella virus 1 | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
dsDNA + ATP + H2O | Chlorella virus Marburg | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus Marburg | - |
i.e. CVM-1 | - |
Paramecium bursaria Chlorella virus 1 | - |
i.e. PBCV-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CVM-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of CVM-1 viral DNA, viral DNA contains 10% N6-methyladenine and 42% 5-methylcytosine, 4fold decreased activity compared to unmodified DNA | Chlorella virus Marburg | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Chlorella virus Marburg | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Chlorella virus Marburg | ? | - |
? | |
additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Chlorella virus Marburg | ? | - |
? | |
PBCV-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of PBCV-1 viral DNA, viral DNA contains N6-methyladenine and 5-methylcytosine, decreased activity compared to unmodified DNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Topoisomerase II | - |
Paramecium bursaria Chlorella virus 1 |
Topoisomerase II | - |
Chlorella virus Marburg |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
30 | - |
assay at | Chlorella virus Marburg |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
8.5 | - |
assay at | Chlorella virus Marburg |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Paramecium bursaria Chlorella virus 1 | |
ATP | - |
Chlorella virus Marburg |