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Literature summary for 5.6.2.2 extracted from
- Impact of the C-terminal domain of topoisomerase IIalpha on the DNA cleavage activity of the human enzyme (2005), Biochemistry, 44, 11546-11554.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and truncation mutant Top2alphaDELTA1175 in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a truncation mutant Top2alphaDELTA1175 cleaved at residue 1175, which shows 2-3fold increased DNA cleavage activity but reduced overall activity, and also a reduced response to anticancer drugs | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amsacrine | - |
Homo sapiens |  |
| CP-115953 | - |
Homo sapiens | |
| EDTA | at 77 mM | Homo sapiens | |
| etoposide | - |
Homo sapiens | |
| additional information | the C-terminus of the enzyme is responsible for sensitivity to anticancer drugs | Homo sapiens | |
| SDS | 0.5% | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsDNA + ATP + H2O | Homo sapiens | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Homo sapiens | ? | - |
? | |
| kinetoplast DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA isolated from Crithidia fasciculata, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms | Homo sapiens | ? | - |
? | |
| additional information | the enzyme reaction is modulated by the C-terminus of the enzyme, which is also responsible for sensitivity to anti-cancer drugs | Homo sapiens | ? | - |
? | |
| negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | coordination between subunits of wild-type and truncation mutant Top2alphaDELTA1175, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Top2alpha | - |
Homo sapiens |
| topoisomerase IIalpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Homo sapiens | |
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Release 2023.1 (January 2023)
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