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Literature summary for 5.6.2.2 extracted from
- Mechanism of action of ferrocene derivatives on the catalytic activity of topoisomerase IIalpha and beta - distinct mode of action of two derivatives (2005), Arch. Biochem. Biophys., 438, 206-216.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azalactone ferrocene | specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.1 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | |
| additional information | inhibitory potency of diverse ferrocene derivatives, overview | Rattus norvegicus | |
| thiomorpholide amido methyl ferrocene | specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.05 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Rattus norvegicus |  |
| Mg2+ | - |
Rattus norvegicus | |
| NaCl | required for DNA cleavage | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes IIalpha and IIbeta from cerebellum by 2 steps of ion exchange chromatography, and immunoaffinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme shows ATPase activity | Rattus norvegicus | ? | - |
? | |
| pBR322 DNA + ATP + H2O | cleavage of pBR322 DNA | Rattus norvegicus | ? | - |
? | |
| supercoiled pRYG DNA + ATP + H2O | relaxation of supercoiled pRYG DNA | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topoisomerase IIalpha | - |
Rattus norvegicus |
| topoisomerase IIbeta | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ATPase assay at | Rattus norvegicus |
| 7.9 | - |
DNA cleavage assay at | Rattus norvegicus |
| 8 | - |
DNA relaxation assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | required for DNA relaxation and ATPase activities | Rattus norvegicus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.05 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | thiomorpholide amido methyl ferrocene | |
| 0.1 | - |
specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.1 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | azalactone ferrocene |
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