Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.2 extracted from

  • Park, S.H.; Lee, C.G.; Namkung, R.; Kim, C.G.; Park, S.D.
    Active DNA topoisomerase II with minimum molecular mass from regenerating rat liver (1994), Biochem. Mol. Biol. Int., 32, 641-650.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Nalidixic acid no inhibition Rattus norvegicus
novobiocin 1 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
173000
-
 bands of MW 55000 and MW 70000 detected by SDS-PAGE represent proteolytic products of the 173000 MW form, however these products retain the enzyme activities. The topoisomerase II consists of different functional domains, the whole enzyme is not required for its activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver regenerating Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
network of DNA rings + ATP + H2O decatenation Rattus norvegicus monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Rattus norvegicus catenated DNA networks + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O relaxation Rattus norvegicus relaxed DNA + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 173000, bands of MW 55000 and MW 70000 detected by SDS-PAGE represent proteolytic products of the 173000 MW form, however these products retain the enzyme activities. The topoisomerase II consists of different functional domains, the whole enzyme is not required for its activity Rattus norvegicus