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Literature summary for 5.6.2.2 extracted from
- Novobiocin affinity purification of a mitochondrial type II topoisomerase from the trypanosomatid Crithidia fasciculata (1989), J. Biol. Chem., 264, 1870-1876.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4'-(9-acridinylamino)methansulfon-m-anisidide | - |
Crithidia fasciculata |  |
| Berenil | - |
Crithidia fasciculata | |
| coumermycin | - |
Crithidia fasciculata | |
| ellipticines | - |
Crithidia fasciculata | |
| Ethidium bromide | - |
Crithidia fasciculata | |
| etoposide | - |
Crithidia fasciculata | |
| NEM | - |
Crithidia fasciculata | |
| novobiocin | - |
Crithidia fasciculata | |
| Teniposide | - |
Crithidia fasciculata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Crithidia fasciculata | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 | Crithidia fasciculata | |
| Mg2+ | required | Crithidia fasciculata | |
| Mg2+ | effective at 0.1-8 mM MgCl2 | Crithidia fasciculata | |
| NaCl | requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 | Crithidia fasciculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
2 * 132000, SDS-PAGE | Crithidia fasciculata |
| 264000 | - |
gel filtration, glycerol density gradient sedimentation | Crithidia fasciculata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Crithidia fasciculata | localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| novobiocin affinity purification | Crithidia fasciculata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication | Crithidia fasciculata | ? | - |
? | |
| network of DNA rings + ATP + H2O | decatenation | Crithidia fasciculata | monomeric DNA circles + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Crithidia fasciculata | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation | Crithidia fasciculata | relaxed DNA + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | ATP-dependent relaxation | Crithidia fasciculata | relaxed DNA + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 132000, SDS-PAGE | Crithidia fasciculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.8 | - |
Crithidia fasciculata |
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