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Literature summary for 5.6.2.2 extracted from
- Purification and characterization of type II DNA topoisomerase from mouse FM3A cells: phosphorylation of topoisomerase II and modification of its activity (1990), Biochemistry, 29, 583-590.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | phosphorylation by protein kinase increases the activity by 8.6fold over that of the unmodified enzyme | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4'-(9-acridinylamino)methansulfon-m-anisidide | epipodophyllotoxin-resistant line, VpmR-5, is not inhibited | Mus musculus |  |
| actinomycins | actinomycin D | Mus musculus | |
| camptothecin | - |
Mus musculus | |
| coumermycin | - |
Mus musculus | |
| Ethidium bromide | - |
Mus musculus | |
| Nalidixic acid | - |
Mus musculus | |
| novobiocin | - |
Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| FM3A cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | dATP can substitute for ATP. ITP is slightly effective at 5-10 mM | Mus musculus | ? | - |
? | |
| network of DNA rings + ATP + H2O | decatenation | Mus musculus | monomeric DNA circles + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Mus musculus | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation | Mus musculus | relaxed DNA + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | two bands of MW 167000 and 151000 are detected by SDS-PAGE | Mus musculus |
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