Any feedback?
Literature summary for 5.6.2.2 extracted from
- Purification and characterization of DNA topoisomerase II from calf thymus associated with polypeptides of 175 and 150 kDA (1986), Eur. J. Biochem., 160, 451-457.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
ATP | relaxation reaction | Bos taurus |  |
| 0.5 | - |
dATP | relaxation reaction | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Bos taurus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essential cofactor for the reaction at concentrations between 0.5-8 mM, optimal concentration: 4 mM | Bos taurus | |
| Mn2+ | can substitute for Mg2+, but only at concentrations between 0.2 and 0.4 mM | Bos taurus | |
| NaCl | catenation and relaxation exhibit a salt optimum at 130 mM. At concentrations below 30 mM and above 200 mM the enzyme is inactive | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
| 175000 | - |
1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
| 300000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, stable for at least 12 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + negatively supercoiled circular DNA | - |
Bos taurus | dADP + phosphate + relaxed circular DNA | - |
? | |
| additional information | - |
Bos taurus | ? | - |
? | |
| network of DNA rings + ATP + H2O | decatenation | Bos taurus | monomeric DNA circles + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Bos taurus | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | in presence of condensing agents like histone H1 or spermidine | Bos taurus | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation | Bos taurus | relaxed DNA + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | Bos taurus | relaxed DNA + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | dATP can substitute for ATP | Bos taurus | relaxed DNA + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7: about 55% of maximal activity, pH 10: about 25% of maximal activity, relaxing reaction | Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
