Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.2 extracted from

  • Kreuzer, K.N.; Jongeneel, C.V.
    Escherichia coli phage T4 topoisomerase (1983), Methods Enzymol., 100, 144-160.
    View publication on PubMed
Search for more literature for 5.6.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for double-stranded DNA is 0.0015 mg/ml, 0.0045 mM nucleotides Tequatrovirus T4

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the products of gene 39, MW 57000, and gene 52, MW 48000, are tightly membrane-associated Tequatrovirus T4 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Tequatrovirus T4
Mg2+ required Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4
48000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4
57000
-
 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Source Tissue

Source Tissue Comment Organism Textmining
additional information E. coli, bacteriophage T4-infected Tequatrovirus T4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme also cleaves single-stranded DNA in a site-specific fashion, but this cleavage is mechanistically distinct from the double-strand DNA cleavage Tequatrovirus T4 ?
-
 ?
additional information cannot supercoil various E. coli plasmid DNAs in vitro Tequatrovirus T4 ?
-
 ?
network of DNA rings + ATP + H2O decatenation Tequatrovirus T4 monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Tequatrovirus T4 catenated DNA networks + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O relaxation Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?
supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?
supercoiled DNA + ATP + H2O pBR322 plasmid DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ?

Subunits

Subunits Comment Organism
hexamer 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE Tequatrovirus T4