Protein Variants | Comment | Organism |
---|---|---|
Y723F | the active site mutant shows abolished formation of Top1 covalent adducts with DNA structures | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | partial inhibition of the Top1-DNAadduct formation upon addition of poly(ADP ribose) polymerase 1 in the absence of NAD+ is shown, whereas in the presence of NAD+ formation of a high molecular weight product, most likely corresponding to poly(ADP) ribosylated Top1-DNA adduct, is observed | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Top1 relaxes supercoiled structures in DNA, thus creating a nick. Top1 cleaves a phosphodiester bond near the 3' end of the nick and forms a covalent bond with the 3' end of the newly formed break. This process involves the catalytic tyrosine residue necessary for nucleophilic attack and disruption of a phosphodiester bond in DNA. and it involves poly(ADP-ribose) polymerase 1, that phosphoribosylates the enzyme and affect binding to DNA, overview. Interaction of human recombinant DNA topoisomerase 1 with linear and circular DNA structures containing a nick or short gap but lacking a specific Top1 recognition site, using circular M13mp19 phage DNA and the 34mer DNA duplex containing uracil in position 16 of one of the duplex strands (U-G) as substrate. Partial inhibition of the Top1-DNAadduct formation upon addition of poly(ADP ribose) polymerase 1 in the absence of NAD+ is shown, whereas in the presence of NAD+ formation of a high molecular weight product, most likely corresponding to poly(ADP) ribosylated Top1-DNA adduct, is observed. Top1 covalent adducts with DNA containing a singlestranded break or a short gap in the absence of a recognition site are also formed with involvement of the catalytic tyrosine residue in the enzyme active center | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase 1 | - |
Homo sapiens |
Top1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Homo sapiens |