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Literature summary for 5.6.2.1 extracted from

  • Kwon, K.; Nagarajan, R.; Stivers, J.T.
    Ribonuclease activity of vaccinia DNA topoisomerase IB: kinetic and high-throughput inhibition studies using a robust continuous fluorescence assay (2004), Biochemistry, 43, 14994-15004.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(1-phenyl-ethylideneamino)-acetic acid 0.05 mM, 53% vTopo RNase activity remaining, 10% cTopo relaxation activity remaining Vaccinia virus
(2-hydroxy-naphthalen-1-yl)-acetic acid 0.05 mM, 48% vTopo RNase activity remaining Vaccinia virus
(3-hydroxy-4-thiocyanato-benzylsulfanyl)-acetic acid 0.05 mM, 45% vTopo RNase activity remaining Vaccinia virus
2-(2-nitro-vinyl)-1H-indole 0.05 mM, 54% vTopo Rnase activity remaining Vaccinia virus
2-(furan-2-carbonyl)-3-(pyridin-2-ylamino)-acrylonitrile 0.05 mM, 28% vTopo RNase activity remaining; 0.05 mM, 7% vTopo RNase activity remaining, 2% cTopo relaxation activity remaining Vaccinia virus
3,5-bis-dimethylaminomethyl-benzene-1,2-diol 0.05 mM, 52% vTopo RNase activity remaining, 30% cTopo relaxation activity remaining Vaccinia virus
3-(5-phenyl-1H-pyrrol-2-yl)-propanoic acid 0.05 mM, 35% vTopo RNase activity remaining Vaccinia virus
3-benzo[1,3]dioxol-5-yl-1,1,1-trihydroxy-2-oxopropanoic acid 0.05 mM, 9% vTopo RNase activity remaining, 8% cTopo relaxation activity remaining Vaccinia virus
3-benzo[1,3]dioxol-5-yl-2-oxopropionic acid potent inhibitor of the enzyme from vaccinia virus, does not inhibit the human enzyme Vaccinia virus
3-[4-(1-ethyl-2-methyl-propenyl)-phenoxy]-benzoic acid 0.05 mM, 33% vTopo RNase activity remaining Vaccinia virus
4,4'-dimethyl-biphenyl-2,5,2',5'-tetraol 0.05 mM, 30% vTopo RNase activity remaining Vaccinia virus
4-(1-methylaminomethylsulfanyl-ethyl)-benzene-1,2-diol 0.05 mM, 27% vTopo RNase activity remaining Vaccinia virus
S-Dimethylaminomethyl-N-(3,3,3-trichloro-1-hydroxy-prop-1-yl)-isothiourea 0.05 mM, 28% vTopo RNase activity remaining Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the vaccinia type I DNA topoisomerase exhibits a strong site-specific ribonuclease activity when provided a DNA substrate that contains a single uridine ribonucleotide within a duplex DNA containing the sequence 5'CCCTU3'. The reaction involves two steps: attack of the active site tyrosine nucleophile followed by nucleophilic attack of the uridine 2'-hydroxyl to release the covalently tethered enzyme Vaccinia virus ?
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?

Synonyms

Synonyms Comment Organism
DNA topoisomerase IB
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Vaccinia virus
vTopo
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Vaccinia virus