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Literature summary for 5.6.2.1 extracted from

  • Perry, K.; Mondragon, A.
    Biochemical characterization of an invariant histidine involved in Escherichia coli DNA topoisomerase I catalysis (2002), J. Biol. Chem., 277, 13237-13245.
    View publication on PubMed
Search for more literature for 5.6.2.1

Protein Variants

Protein Variants Comment Organism
H365A whereas wild type enzyme shows pH-dependent cleavage of oligonucleotides, cleavage by the mutant enzyme is pH-independent. Cleaves DNA with a slower rate than the wild-type enzyme, but can cleave more substrate overall, mutant enzyme has a lower DNA binding affinity than the wild-type enzyme, mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli
H365D | mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli
H365E | mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli
H365N mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli
H365Q whereas wild type enzyme shows pH-dependent cleavage of oligonucleotides, cleavage by the mutant enzyme is pH-independent. Cleaves DNA with rates similar to the wild-type enzyme, mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli
H365R | mutant shows a shift in maximal relaxation to above pH 8.5, mutant enzyme is less able than the wild type enzyme to relax negatively supercoiled DNA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled DNA
-
 Escherichia coli ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 wild-type enzyme, relaxation activity Escherichia coli
8.5
-
 mutant enzymes H365Q, H365A, H365N, H365D, H365E and H365R, relaxation activity Escherichia coli